Ionkromatografer til rutinebrug og forskningsapplikationer: fleksible, pålidelige og lette at bruge.
Den første ionkromatograf blev føjet til Metrohms portefølje af analytisk kemisk instrumentering i 1987. Siden da har Metrohm udviklet og fremstillet avancerede IC-løsninger, fra simple opsætninger til forskningslaboratorier til fuldautomatiserede, højtydende IC-systemer og kombinerede teknikker såsom IC-MS eller IC-ICP/MS såvel som selve ionkromatografiens hjerte: separationskolonnerne.
Lær mere om Metrohm IC i vores blog:
Ionkromatografer er analytiske instrumenter, der bruges til at adskille og kvantificere ioner i væskeprøver. Følgende trin udføres af et ionkromatografi-instrument fra Metrohm:
Lær mere om ionkromatografi:
Webinar: An introduction to ion chromatography: Principles and applications
Takket være det brede udvalg af ionkromatografikolonner fra Metrohm kan enhver applikation udføres ved hjælp af Metrohm IC-systemer. Metrohm IC-instrumenter kan bruges til miljø-, farmaceutiske samt vand- og spildevandsanalyser, i fødevare- og drikkevareindustrien, kemi- og energiproduktionsindustrien samt forskning.
Separationskolonnerne dækker de mest almindelige separationsmekanismer, dvs. ionbytning, ionudelukkelse og ionpardannelse og discipliner inden for ionkromatografi:
Eksempler på IC-applikationer:
Lægemiddelmonografier, assays og urenhedsvurderinger
Bestemmelse af adsorberbare organisk bundne halogener (DIN 38409-59)
Et frisk kig på mad- og drikkevareanalyse (e-book)
Forenklet sulfitbestemmelse i fødevarer og drikkevarer
Robust multiparameteranalyse af modermælkserstatninger og tilskudsblandinger
Du kan downloade over 1.700 applikationsdokumenter i Application Finder. Du kan finde flere oplysninger i vores blog, webinarer, eller i Produktets HjælpeCenter..
Lokale eksperter kan også kontaktes for fejlfinding og til at løse komplekse analytiske problemer – enten på stedet eller i et af deres globale applikationslaboratorier.
Ja, overvågning af PFAS (per- og polyfluoralkylstoffer) er en enkel og bredt anvendt procedure til ionkromatografi i kombination med pyrohydrolytisk forbrænding, kendt som Combustion Ion Chromatography (CIC). CIC muliggør bestemmelse af total og organisk fluor i en lang række prøver, såsom spildevand eller tekstiler.
CIC kan anvendes til PFAS-screening via AOF (Adsorberbart Organisk Fluor). CIC-metoden indebærer adsorption af organofluorforbindelser på aktivt kul, forbrænding ved høj temperatur (1050 °C) for at omdanne organisk bundet fluor til hydrogenfluorid, samt absorption af forbrændingsgasserne (HF) i vand.
IC bruges til at kvantificere fluoridioner, hvilket indirekte indikerer tilstedeværelsen af PFAS. Denne tilgang er standardiseret (f.eks. U.S. EPA Metode 1621, DIN 38409-59, ISO/DIS 18127) og velegnet til ikke-målrettet screening af PFAS i vandige prøver.
Applikationsnote: Monitoring PFASs in water sources with CIC
White Paper: Adsorbable organic fluorine (AOF) for screening of PFAS in waters
Brug af ionkromatografi koblet med tandem massespektrometri er en pålidelig og valideret metode (DIN 38407-53) til at kvantificere kortkædede og ultrakortkædede PFAS, såsom trifluoreddikesyre (TFA), og understøtte miljøovervågning.
Kortkædede og ultrakortkædede PFAS (S- og US-PFAS) er nedbrydnings- og erstatningsprodukter af langkædede PFAS med færre carbonatomer. På grund af deres høje vandopløselighed og persistens kan ultrakort- og kortkædede PFAS nemt migrere til drikkevandskilder. TFA er et fremtrædende eksempel på en ultrakortkædet PFAS. Dens lille molekylstørrelse gør den meget mobil og vanskelig at fjerne gennem konventionel vandbehandling.
Lær mere:
Applikationsnote: IC-MS/MS analysis of trifluoroacetic acid according to DIN 38407-53
Ja, ionkromatografi er blevet bredt accepteret af pharmaindustrien og regulerende myndigheder til et udvidet udvalg af anvendelser, såsom vandkvalitet og kemisk analyse. I de senere år er IC blevet kvalificeret til at analysere lægemidler.
United States Pharmacopeia-National Formulary (USP-NF), den førende standardiseringsorganisation for (bio)farmaceutiske produkter, har øget IC's potentiale gennem deres moderniseringsinitiativer og etableret det som en passende og valid teknik i deres kompendiale generelle kapitler (f.eks. USP Generelt Kapitel <1065> Ion Chromatography), analyser og urenhedstests.
Blog: Ion chromatography for the pharmaceutical industry
Blog: Applying USP validated methods for separation column equivalency
White Paper: When HPLC fails: IC in food, water, and pharmaceutical analysis
Flyer: Bring your USP methods up to date (8.000.5436, PDF, 900 KB)
Applikationsspecifikke kriterier er de vigtigste faktorer for din beslutningstagning. Husk følgende:
Vores IC-eksperter hjælper dig gerne med alle dine spørgsmål:
Det afhænger af instrumentet. For eksempel kan 940 Professional IC Vario-systemerne fra Metrohm opgraderes når som helst. Disse systemer er designet med en modulær og meget fleksibel arkitektur, som gør det muligt for brugerne at tilpasse dem til skiftende analytiske krav. Tilføj efter behov, for eksempel:
Modularitet sikrer, at systemet forbliver fremtidssikret og kan opgraderes for at imødekomme fremtidige krav.
Brochure: 940 Professional IC Vario and 942 Extension Module Vario (8.940.5003, PDF, 2.3 MB)
Yes, Metrohm IC systems can be used for applications compliant with EPA 300.0, EPA 300.1 parts A and B, or ISO 14911.
With the 930 Compact IC Flex, equipped with the 948 Continuous IC Module for automatic hydroxide eluent generation, an MiPT (Metrohm intelligent Partial Loop Injection Technique) setup for single-standard calibration, and the high-capacity Metrosep A Supp 21 column, Metrohm provides a reliable and economic setup to analyze water samples according to EPA Method 300.0 as well as EPA 300.1 Parts A and B in a single IC run.
Application Note: Drinking water quality by EPA 300.1
White Paper: Determination of anions in tap water in accordance with US EPA Method 300
Utilizing a Metrosep C 4 – 150/4.0 or a Metrosep C6 – 150/4.0 column in conjunction with a 940 Professional IC Vario allows for compliance with the requirements specified in ISO 14911. Furthermore, incorporating an 858 Professional Sample Processor and Metrohm Inline Sample Preparation options can enhance the speed of analysis while reducing manual workload.
The ideal solution for high-throughput labs is a reliable, accurate IC system consisting of the following components:
Blog: Save time and money with Metrohm Inline Sample Preparation solutions
Blog: Boost your environmental analysis with Metrohm automated ion chromatography
Yes, for Metrohm IC instruments, eluent generation can be automated.
The 948 Continuous IC Module, CEP is the automated solution for the production of hydroxide eluents. With a direct connection to a water purification system (e.g., ELGA® PURELAB® flex 5/6), the IC can be operated unattended 24/7.
The 941 Eluent Production Module enables the production of eluents of any composition and concentration such as salts or diluted acids. Thanks to Inline Dilution and the patented Dosino technology, up to four eluents can be produced in parallel with just one module. Combined with a direct connection to a water purification system (e.g., ELGA® PURELAB® flex 3+), the IC can be operated unattended 24/7.
Webinar: Automatic hydroxide eluent preparation: Overcoming inefficiencies in anion analysis
Yes, IC can perform gradient elution. Metrohm offers several systems to support gradient elution:
Eluents are mixed using two or more high-pressure pumps. The Metrohm high-pressure gradient (HPG) system (e.g., 940 Professional IC Vario TWO/SeS/PP with IC Conductivity Detector and a 942 Extension Module Vario HPG for 3 eluents) uses three high-pressure pumps to mix eluents under pressure, allowing for various gradient profiles such as linear, stepwise, concave, and convex.
This system is particularly suitable for complex separations, including anions, cations, sugars, amino acids, and other polar substances.
Low-pressure gradients are used in ion chromatography to improve peak separation for complex applications and matrices.
Typical area of application:
A single high-pressure pump aspirates eluents through a mixing capillary, with a low-pressure valve controlling the mixing ratio. The setup is simple, solvent-stable, and it supports a wide range of gradient profiles.
A compact and cost-effective example is the low-pressure (LPG) system from Metrohm (e.g., 940 Professional IC Vario ONE/SeS/PP/LPG).
Brochure: Gradients in ion chromatography (8.000.5131, PDF, 2.2 MB)
A Dose-in gradient is highly flexible and can be retrofitted to any Metrohm IC system (e.g., the combination of 940 Professional IC Vario and 800 Dosino for Dose-in Gradients).
It works in the low-pressure range using a high-pressure pump and a 6-port adapter to dose up to five eluents. This system supports both linear and stepwise gradients and is compatible with organic solvents. It is easy to install and operate, making it a practical choice for laboratories requiring adaptable and straightforward gradient capabilities.
The hydroxide gradient by eluent generation creates hydroxide eluents electrochemically from a concentrated stock solution. This is done with the help of the eluent generator (948 Continuous IC Module, CEP) together with a supply of ultrapure water, e.g., by a water purification system. The eluent and gradient generation process happens on-demand and inline, and the generated eluent is then delivered by a high-pressure pump. It has a higher precision and less noise than a high-pressure gradient.
Application Note: Water quality testing with EPA 300.1
Application Note: Trace haloacetic acids, dalapon, and bromate measurement in drinking water
The replacement as well as service intervals highly depend on the samples that are measured. Usually, regular maintenance can help to improve the lifetime of the suppressor and the column.
Further tips:
Learn more about the maintenance intervals for IC instruments
Yes, filtering samples for ion chromatography (IC) is generally recommended and often necessary.
Why do IC samples need to be filtered?
How are IC samples typically filtered?
Learn more about Inline Ultrafiltration in our blog:
Blog: Protecting IC systems with Metrohm Inline Ultrafiltration and Inline Dialysis
Yes, you can run both anions and cations on the same IC system. However, you require an anion exchange column for anion separation and a cation exchange column for cation separation.
If you use a single-channel ion chromatograph, such as 930 Compact IC Flex SeS/PP, you can run anions and cations consecutively. The 930 Compact IC Flex SeS/PP is the intelligent Compact IC instrument with sequential suppression and a peristaltic pump for suppressor regeneration. The instrument can be used with any separation and detection methods.
Typical areas of application include:
However, single-channel ion chromatographs need to be cleaned thoroughly in between the measurements to avoid crosscontamination. Using a dual-channel system is the better solution, as you can run anions and cations in parallel.
Metrohm offers various ProfIC Vario systems with two analysis channels, enabling fully automatic determination of anions and cations, both in parallel and also completely independently of one another as a time-saving feature.
Typical areas of application include:
Application Note: Metrohm IC Driver for OpenLab CDS: Anion and cation analysis in a soft drink
To analyse anions and cations with suppressed conductivity detection, you need an IC system that is equipped with an anion and a cation separation column, two conductivity detectors, and the capability for anion suppression.
Metrohm offers various systems with sequential suppression, conductivity detection, and two analysis channels, enabling fully automatic determination of anions and cations, both in parallel and also completely independently of one another.
Typical areas of application include:
The modular system is upgradable. In case you need cation suppression, an extension module can be added to the system. The 942 Extension Module Vario SeS/PP is an extension module for the Professional IC Vario line of instruments. It allows the integration of a complete sequential suppression with a peristaltic pump for suppressor regeneration.
Typical applications include:
Application Note: Metrohm IC Driver for OpenLab CDS: Anion and cation analysis in a soft drink
Metrohm IC systems are controlled with our proprietary ion chromatography software MagIC Net. MagIC Net is a simple and intuitive software that controls the components of the intelligent Metrohm ion chromatography systems as well as their peripheral devices for Liquid Handling and automation. MagIC Net meets all FDA and GLP requirements.
In addition, Metrohm IC systems can be fully controlled by OpenLab CDS from Agilent and EmpowerTM from WatersTM. They enable hyphenation of Metrohm IC with single quadrupole mass spectrometers and their operation in a single software. For hyphenation with ICP-MS, Metrohm developed a solution for single-software use of Metrohm IC and Agilent ICP-MS in ICP-MS MassHunter.
Brochure: MagIC Net 4 (8.102.5010, PDF, 1.4 MB)
Brochure: IC Driver for Agilent OpenLab CDS (8.102.5007, PDF, 1 MB)
Brochure: Metrohm meets Empower 3 (8.102.5004, PDF, 213 KB)
Brochure: IC Driver for ICP-MS MassHunter Software (8.000.5496, PDF, 1.25 MB)
Yes, you can use OpenLab CDS to control ion chromatographs from Metrohm. Supported Metrohm IC instruments include:
Additionally, you can control and operate both Metrohm IC and Agilent single quadrupole MS instruments within one software environment (OpenLab CDS), enabling IC-MS hyphenation.
Yes, OpenLab CDS can handle data from both Metrohm IC and Agilent MS instruments together when they are integrated in a hyphenated setup (IC-MS).
Data handling in OpenLab CDS supports synchronized acquisition and processing of data from both instruments. You can view chromatograms and mass spectra in a single interface, perform peak identification and quantification using both IC and MS data, and generate combined reports that include results from both systems.
The integration streamlines workflows for trace analysis, speciation, and complex matrix investigations. It eliminates the need to manually merge data from separate software platforms.
Requirements: You need the Metrohm IC Driver for OpenLab CDS and a compatible version of OpenLab CDS (2.3–2.8) including an Agilent license for non-Agilent Instrument Connection (for CDS 2.4+).
Yes, when Metrohm IC instruments are integrated into OpenLab CDS using the Metrohm IC Driver, the audit trail functionality remains fully supported.
Requirements: A non-Agilent Instrument Connection license is required for OpenLab CDS 2.4+.
Yes, the MagIC Net software is audit-trail capable.
MagIC Net:
When 21 CFR Part 11 is activated in MagIC Net, all previous versions of the modified data are stored in the database.
MagIC Net supports 21 CFR Part 11 compliance, which includes audit trail requirements for electronic records and electronic signatures. This ensures traceability, accountability, and data integrity – key requirements for regulated environments like pharmaceutical or food testing labs.
Brochure: MagIC Net – Intelligent Software for Ion Chromatography (8.102.5010, PDF, 1.5 MB)
Metrohm IC systems are compliant with 21 CFR Part 11 when operated with the software MagIC Net, OpenLab CDS, or EmpowerTM. The compliance governs electronic records and electronic signatures in FDA-regulated environments. Compliance usually includes features like secure user authentication, audit trails, electronic signatures, and data integrity controls.
Learn more about compliance in regulated environments:
Blog: Ensuring and certifying quality and compliance in regulated environments
Yes, measurement data can be exported to LIMS (Laboratory Information Management Systems) via MagIC Net. This is typically done via standardized data formats or direct integration protocols, which are often mentioned in system documentation or specifications.