Temperature plays an important role, especially during an analysis series. Each solution has a specific coefficient of thermal expansion. The coefficient is defined as such:

#### V = V_{0} ∙ (1 + γ ∙ ∆T)

Where **V** corresponds to the volume at a certain temperature, **V _{0}** to the nominal volume,

**γ**to the coefficient of thermal expansion (in 10

^{-3}K

^{-1}), and

**∆T**corresponds to the temperature difference between the temperature of the nominal volume (V

_{0}) and the measured temperature (in K).

Depending on the thermal expansion coefficient (γ), keeping the temperature of the solution constant could be a critical point. For example, n-hexane has a coefficient of 1.35. Assuming the solution is 1.000 L at 20 °C and the ambient surroundings are 25 °C, the volume of the solution is 1.007 L at this temperature. This corresponds to an error of 0.7%.

Therefore, the coefficient of thermal expansion for a solution may be an important enough factor to regulate the temperature in the laboratory to obtain reproducible results.