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Metrosep C Supp 2 - 250/4.0

Metrosep C Supp 2 - 250/4.0

6.01053.430

Metrosep C Supp 2 - 250/4.0

en

6.01053.430

Feature overview

The longest separation column in the Metrosep C Supp 2 product range is the Metrosep C Supp 2 - 250/4.0. The Metrosep C Supp 2 separation material is based on a polystyrene-divinylbenzene copolymer with carboxyl groups. Thanks to the optimized sodium/ammonium separation of this separation material, this column is perfectly suitable for determination of the smallest concentrations of ammonium in addition to a large amount of sodium. The column is used with sequential suppression. So it is particularly suitable for determining concentrations in the middle µg/L range and lower.

Tech specs

ApplicationRange

Applications:
 

  • Li+, Na+, K+, Mg2+, Ca2+, Sr2+, Ba2+, NH4+
  • Samples with low concentrations
  • Good Na+/NH4+ separation
  • Low detection limits
  • Transition metals
  • Excellent peak form
  • Matrix with high pH
Organic Modifier

Eluent: 0 - 100% acetone and acetonitrile (no alcohol)
Sample: 0 - 100% acetone, acetonitrile, and alcohols

pH range

Eluent: 0 - 12
Sample: 0 - 14

Regeneration

Note:

Ensure that the maximum pressure is never exceeded during regeneration.
If the pressure becomes too high, reduce the flow rate.

Regeneration:

Disconnect the column outlet from the downstream functional units such as suppressor or detector. Instead, collect the flow of liquid in a beaker.
Rinse the column with ultrapure water before and after regeneration.

Depending on the type of contamination, proceed in accordance with one of the following instructions:

Organic contamination:

Regenerate the column in the opposite direction to the flow at 1 mL/min with the following solutions in succession:

1 h with ultrapure water
1 h with acetonitrile-water mixture (40:60)
1 h with ultrapure water

Inorganic contamination:

Regenerate the column in the opposite direction to the flow at a flow rate of 1 mL/min over 1 h with 50 mmol/L nitric acid
.

StandardEluent

Nitric acid eluent (standard eluent)

Nitric acid (c = 1 mol/L): 5.0 mmol/L; 10 mL/2 L
Rubidium (from rubidium nitrate): 50 µg/L Rb+; 172.5 µg/2 L (RbNO3)

Storage

In standard eluent at ambient temperature.

Capacity

58 µmol (K+)

Housing materialPEEK
Carrier materialPolystyrene-divinylbenzene copolymer with carboxyl groups
Particle size5 µm
Standard flow1.0 mL/min
Maximum pressure25 MPa
Maximum flow2.0 mL/min
Column dimensions250 x 4.0 mm
Temperature range10–60 °C
Standard temperature40 °C

Product Family

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Documents

Find your certificate

Where to find the serial and article numbers?

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    • Threaded stopper
    • 6.2744.060
    • For UNF 10/32. Stopper for IC, e.g. for the sealing of columns.
    • <p>For UNF 10/32. Stopper for IC, e.g. for the sealing of columns.</p>
    • PCS
    • 2
    • Threaded stopper
    • 6.2744.060
    • For UNF 10/32. Stopper for IC, e.g. for the sealing of columns.
    • <p>For UNF 10/32. Stopper for IC, e.g. for the sealing of columns.</p>
    • PCS
    • 2
    • Metrosep C Supp 2 Guard/4.0
    • 6.01053.500
    • The Metrosep C Supp 2 Guard/4.0 contains the C Supp 2 column material and is used to protect Metrosep C Supp 2 cation columns. Particles and contamination are reliably retained, considerably prolonging the service life of the analytical separation column. The Metrosep C Supp 2 Guard/4.0 also works based on the "On Column Guard System" and is screwed directly onto the separation column with virtually no dead volume.
    • <p>The Metrosep C Supp 2 Guard/4.0 contains the C Supp 2 column material and is used to protect Metrosep C Supp 2 cation columns. Particles and contamination are reliably retained, considerably prolonging the service life of the analytical separation column. The Metrosep C Supp 2 Guard/4.0 also works based on the &#34;On Column Guard System&#34; and is screwed directly onto the separation column with virtually no dead volume.</p>
    • PCS
    • 1