Ethanol content in wine by near-infrared spectroscopy
AN-NIR-147
2025-12
de
Rapid determination of alcohol levels in wine with NIRS
Ethanol content can be quickly and easily determined in different wines during fermentation and afterwards for quality control using near-infrared spectroscopy.
Ethanol content is an important parameter to determine in wine—during the fermentation process, for quality control, and for other legal requirements of alcoholic beverages. Traditional time-consuming methods like gas chromatography (GC) can be used for the determination of ethanol content in wine. However, measuring the alcohol content in wine can be done easily without sample preparation by using near-infrared spectroscopy (NIRS).
NIRS is a fast, easy to use, and chemical-free analysis method. The NIRS solution can be used either atline during the wine fermentation process or in a quality control laboratory.
?qlt=85&ts=1764833161339&dpr=off&bfc=on "OMNIS NIR Analyzer Liquid and OMNIS Sample Robot S – WSM (1T/2P).")
Samples of red wine and white wine with varying alcohol content were measured on an OMNIS NIR Analyzer Liquid (1000–2250 nm). Measurements were performed in transmission mode with a 2 mm flow-through cell and corresponding holder for flow-through cells. The built-in peristaltic pump on the OMNIS Sample Robot S was used for liquid transfer (Figure 1). OMNIS Software was used for all data acquisition and prediction model development.
| Equipment | Article number |
|---|---|
| OMNIS NIR Analyzer Liquid | 2.1070.0010 |
| NIRS 12.5 mm quartz cuvette flow 2 mm | 6.7401.320 |
| Holder OMNIS NIR, flow-through cells | 6.07401.100 |
| OMNIS Stand-Alone license | 6.06003.010 |
| OMNIS Stand-Alone: 1 instrument license | 6.06002.010 |
| Software license Quant Development | 6.06008.002 |
| OMNIS Sample Robot S – WSM (1T/2P) | 2.1010.1120 |
| OMNIS Sample rack, 25 x 75 mL (PP) | 6.02041.040 |
| Gripper fingers 28-48 mm | 6.02601.040 |
The obtained NIR spectra of wine (Figure 2) were used to create a prediction model for ethanol content. A leave one out validation procedure was applied. A correlation diagram showing the relation between the NIR prediction and the reference values is shown in Figure 3 together with the respective figures of merit (FOM).
?qlt=85&ts=1764833788395&$orig-5000$&dpr=off&bfc=on "NIR spectra of different wine samples measured on an OMNIS NIR Analyzer Liquid with a 2 mm flow-through cell.")
Result ethanol content in wine
| R2 | SEC (% vol) | SECV (% vol) |
|---|---|---|
| 0.995 | 0.080 | 0.082 |
This Application Note shows the feasibility of using NIR spectroscopy for testing ethanol content in wine. The analysis can be conducted within seconds. No chemicals are used, saving costs. Automation possibilities with the OMNIS platform allow the unattended measurement of up to 175 samples.
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