氮含量的测定
AN-T-195
2020-12
zh
Kjeldahl determination according to USP general chapter <461>
凯氏定氮法用于测定有机和无机样品中的氮含量。凯氏定氮法包括三个步骤：消化、蒸馏和滴定。在催化消化步骤中，有机氮1 转化为铵。在蒸馏步骤前加入氢氧化钠，将铵转化为氨。通过蒸汽蒸馏，氨被转移到装有吸收剂（如硼酸）的接收容器中。最后，用硫酸滴定分离出的氨。 样品中的蛋白质含量也可通过凯氏定氮法测定。USP 介绍了使用凯氏定氮法测定有机产品中氮含量的滴定方法。本应用说明介绍了肝素钠中氮含量的测定方法。 ______________________ 1 凯氏定氮法不适用于所有含氮化合物；硝基和偶氮基团以及环中的氮不包括在内。
The Kjeldahl method is used to determine the nitrogen content in organic and inorganic samples. The Kjeldahl analysis consists of three steps: digestion, distillation, and titration. During the catalytic digestion step, organic nitrogen (except nitro- and azo-groups and nitrogen in rings) is converted into ammonium. Sodium hydroxide is added just before the distillation step in order to convert the ammonium into ammonia. Through steam distillation, the latter is transferred into the receiver vessel containing an absorbing agent (e.g., boric acid). Finally, the separated ammonia is titrated with sulfuric acid.
Protein content in samples can also be determined from the nitrogen content obtained by Kjeldahl setup. USP<461> describes the titration method to determine nitrogen content in organic products using Kjeldahl nitrogen setup. This Application Note illustrates nitrogen determination in heparin sodium.
The analysis is demonstrated on heparin sodium. An appropriate amount of heparin sodium is weighed accurately and transferred into a clean 2-neck round bottom flask. Sodium sulfate, copper sulfate, and sulfuric acid are added for the digestion process. The content is gently heated below the boiling point until the frothing ceases. It is then heated again at a higher temperature until the content boils and the solution becomes brown. The contents are cooled and carbon dioxide-free water is added carefully while thoroughly mixing. Sodium hydroxide solution is added through the side-neck of the round bottom flask. Granulated zinc is added and the flask is connected immediately to the Kjeldahl nitrogen distillation setup. The outlet of the setup is put into a solution of boric acid. The distillation is carried out until approximately 80% of the total volume is transferred into the boric acid solution.
The analysis is performed automatically on a Titrando system consisting of a 905 Titrando. The Unitrode is used for the indication of the titration curve.
The prepared sample is titrated potentiometrically against standardized sulfuric acid until after the first equivalence point.
Sharp titration curves are obtained where the equivalence point is reliably determined by the Touch Control or tiamoTM.
The determined nitrogen content of heparin sodium is 1.581% (SD(rel) = 1.48%, n = 5), which is within the nitrogen content specified by USP (1.3% to 2.5%) for heparin sodium.
This method shows the possibility to determine the nitrogen content in various kinds of samples automatically, accurately, and reliably by titration according to the USP general chapter <461>.
Aside from heparin sodium, the following compounds can also be analyzed with this method:
- Antithrombin III Human
- Beta Glucan
- Cellulose sodium phosphate
- Chlorophyllin copper complex sodium
- Colloidal oatmeal
- Copovidone
- Crospovidone
- Dalteparin sodium
- Dextran 1
- Dextrin
- Dihydroxyaluminum aminoacetate,
- Enoxaparin sodium etc.
- Guar gum
- Mecamylamine Hydrochloride tablets
- Melphalan
- Polyvinyl acetate dispersion
- Povidone iodine
- Povidone
- Psyllium hemicellulose
- Pullulan
- Racemic calcium pantothenate
- Ralbumin human
- Scaffold bovine dermis
- Spirulina tablet
- Taurine
- Thioguanine
- Trehalose
- Wheat bran
- Zein
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