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Speciation analysis is an important tool in analytical chemistry giving information about the quantitative distribution of different oxidation states of one and the same metal ion. The speciation of iron(II) and iron(III) (Fe 2+/Fe 3+) is achieved by ion chromatographic separation of their anionic dipicolinic acid complexes. Afterwards, post-column reaction with 4-(2-)pyridylazo-resorcinol (PAR) allows VIS detection at 510 nm.

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