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For milk powder producers, final product control is essential to meet strict regulatory standards, guarantee comprehensive quality assurance, ensure consistent nutritional quality, and extend shelf life. These are all especially important for infant formula and dairy ingredients used in sensitive applications.

Near-infrared spectroscopy (NIRS) is a fast, reagent-free method for measuring key quality parameters such as moisture, protein, lactose, and fat content directly in milk powder. The NIRS solution requires no sample preparation, enabling real-time monitoring either in the lab or directly on the production line. This allows producers to react quickly to process variations, minimize waste, and maintain product integrity batch after batch.

 

Figure 1. OMNIS NIR Analyzer Liquid/Solid.

More than 600 samples of powdered milk from different suppliers were analyzed on an OMNIS NIR Analyzer (Figure 1). The different milk powders were placed into an OMNIS sample cup and analyzed in diffuse reflection mode. To include sample variety, the sample rotated during measurement to collect spectra from different locations. The automatically averaged spectra were used for model development. Reference values were obtained by official methods, e.g., AOAC 927.05 (moisture), AOAC 939.02 (protein), and AOAC 932.06 (fat). For the lactose content determination, a phenol-sulfuric acid method was used. 

The obtained NIR spectra (Figure 2) were used to create prediction models for the different reference parameters. An external validation set was used to verify the predictive performance of the calculated prediction models. Correlation diagrams which display the relation between the NIR prediction and the reference values are shown in Figures 3–6 together with the respective figures of merit (FOM).

 

Figure 2. NIR spectra of milk powder samples. Data was obtained with an OMNIS NIR Analyzer. Spectra shown in blue have been used to calibrate the model, while green spectra have been used for validation.

Result moisture in milk powder

Figure 3. Correlation diagram and the respective FOMs for the prediction of moisture content in milk powder using an OMNIS NIR Analyzer. Reference values were obtained according to AOAC 927.05.

Result protein in milk powder

Figure 4. Correlation diagram and the respective FOMs for the prediction of protein content in milk powder using an OMNIS NIR Analyzer. Reference values were obtained according to AOAC 939.02.

Result fat in milk powder

Figure 5. Correlation diagram and the respective FOMs for the prediction of fat content in milk powder using an OMNIS NIR Analyzer. Reference values were obtained according to AOAC 932.06.

Result lactose in milk powder

Figure 6. Correlation diagram and the respective FOMs for the prediction of lactose content in milk powder using an OMNIS NIR Analyzer.

This Application Note presented the analysis of milk powder using NIR spectroscopy. Models for several quality parameters (fat, protein, lactose, and moisture content) were created. Independent validation samples confirmed the robustness and reliability of the models, with high correlation coefficients and low prediction errors achieved across all parameters. Notably, the dataset included samples from diverse global origins, capturing a broad range of product variability. This study shows that NIRS can be successfully integrated into the quality control workflow for dairy powder analysis.

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