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Succinylcholine is a short-term paralyzing agent used e.g., for tracheal intubation. Choline is a building block of the drug and needs to be determined as an impurity. USP applies cation chromatography with conductivity detection after suppression. Eluent composition and column type do not exactly comply with the USP method. However, the results fulfill the respective requirements. The choline concentration of the sample is out of USP specifications.