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Penicillin and its related antibiotics are used to prevent and treat a number of bacterial infections, such as respiratory tract infections, urinary tract infections, meningitis, etc. It may also be used to prevent group B streptococcal infection in newborns. The β-lactam ring of penicillin binds to the enzyme DD- transpeptidase, which prevents the formation of cross links during cell wall formation of new bacterial cell, i.e., the division of bacterial cells.
USP<425> describes a back-titration method to determine the assay of pharmacopeial penicillin antibiotic drugs and their dosage forms by iodometric titration. The method is illustrated by determining the aminopenicillin content in an ampicillin capsule.


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