High-pressure gradients combine the advantages of weak and concentrated eluents. Weak eluents promote the separation of the components that elute early and in close proximity to one another; in contrast to this, concentrated eluents accelerate the analysis of the components that are stronger retained at the column.
The high-pressure gradient used in this application makes it possible to separate 15 organic acid anions in a single run. The blank subtraction option in the MagIC Net software simplifies the allocation of the peaks and with it the quantification.