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Cell density and growth potential can be determined with the aid of in-situ near-infrared analysis of mammalian cell cultures in real time. The mammalian cell cultures are enriched thereby in a bioreactor with microcarrier cultures. A stable model made up of several passes must be developed in order to ensure that the variability between the individual samplings is taken into account.

NIR spectroscopy can monitor bioreactors "live" and in situ. It is therefore an important instrument in Process Analytical Technology (PAT).

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Metrohm AG

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9100 Herisau

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