Determination of assay of Vitamin C according to USP <580>

Vitamin C, also known as ascorbic acid and L-ascorbic acid, is an essential nutrient involved in the repair of tissues and the enzymatic production of certain neurotransmitters. It is required for the functioning of several enzymes and immune functions. It is also an important antioxidant. It is found in food and used as a dietary supplement. Vitamin C is mainly used to treat scurvy.

USP general chapter <580> describes a titration technique to determine the assay of Vitamin C as ascorbic acid, sodium ascorbate, and calcium ascorbate dehydrate or their mixture in finished dosage forms as capsules, tablets, and oral suspensions. This Application Note details Vitamin C determination in water-soluble vitamin tablets.