In contrast to the routine way of evaluating carryover effect by injecting a blank solution after sample analysis, the use of isotopically enriched standards identified significant analyte carryover in the present method.
Extensive experiments were carried out to systematically identify the source of the carryover and to eliminate the problem; the separation column was found to be the exclusive source. More than 95% of the analyte carryover was eliminated by reducing the length of the column.
Referring to the results of their study relating to carryover effects in chromatography and how to assess them, Wolle et. al. point out that
"this introduces additional dimension for the use of enriched isotopes as diagnostic tool in identifying error prone steps in chromatographic methods."
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