Separation of sugars and sugar acids using low-pressure gradient

A low-pressure gradient enables the separation of sugar acids strongly retained on the column and sugars within an appropriate analysis time. The saccharides are separated on a column of the Metrosep Carb 2 - 250/4.0 type with subsequent pulsed amperometric detection (PAD). Galactose and arabinose are not completely separated under the selected conditions.