Determination of water in lyophilized sample via oven method

Water or moisture determination in lyophilized samples is essential to ensure product quality and shelf life. Many lyophilized samples must be analyzed in prepackaged vials with septum seals. Metrohm has the ability to modify the
sample rack and oven block of its oven sample processor to accommodate varying vial dimensions. In this study, a lyophilized sample was analyzed by coulometric Karl Fischer titration via the oven technique to determine moisture content. The sample was analyzed at both 110oC and 120oC under nitrogen in sealed vials provided by the customer.

Keywords: lyophilize, drug, API