Bethanechol and HPTA (2-hydroxy-propyl-trimethyl ammonium) besides sodium and calcium (Metrosep C 6 - 250/4.0)

Bethanechol is a pharmaceutical compound which is used to treat urinary retention. This API (active pharmaceutical ingredient) can be determined by cation chromatography with direct conductivity detection. A good separation is achieved between bethanechol and its degradation product 2-hydroxy-propyl-trimethyl ammonium (HPTA) and the standard cations. Peak shape and resolution meet the USP requirements for bethanechol.