Metrosep A Supp 10 - 75/4.0

Metrosep A Supp 10 - 75/4.0
Order number: 6.1020.070

The Metrosep A Supp 10 - 75/4.0 separation column is based on a high-capacity polystyrene-divinylbenzene copolymer with a particle size of only 4.6 µm. Under standard conditions, phosphate elutes between nitrite and bromide. Currently, applications can be optimized by modifying temperature, composition of the eluent and flow.

The capacity of the A Supp 10 - 75/4.0 has been optimized with respect to two aspects: Matrix and speed. Baseline separation is achieved in samples with high ionic strength, e.g., for phosphate in cola beverages. Even in the presence of large quantities of nitrate and sulfate, the analysis time remains less than 7.5 minutes. High sample throughput is also of great importance in air analytics.

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Carrier material Polystyrene-divinylbenzene copolymer with quaternary ammonium groups
Column capacity

22 µmol (Cl)

 
Column dimensions 75 x 4.0 mm
Eluents

Carbonate eluent
(
standard eluent)

Sodium hydrogen carbonate: 5.0 mmol/L; 840 mg/2 L
Sodium carbonate: 5.0 mmol/L; 1,060 mg/2 L
(column temperature 45 °C)

Cola eluent

Sodium hydrogen carbonate: 0.4 mmol/L; 67 mg/2 L
Sodium carbonate: 8.0 mmol/L; 1,695 mg/2 L
(column temperature 30 °C)

Carbonate eluent modified:

Sodium hydrogen carbonate: 5.0 mmol/L; 840 mg/2 L
Sodium carbonate: 5.0 mmol/L; 1,060 mg/2 L
Sodium perchlorate: 5 µmol/L; 1.2 mg/2 L
(room temperature)
Housing material PEEK
Max. pressure 25 MPa
Maximum flow 2.0 mL/min
Organic modifier 0 - 100%
Particle size 4.6 µ (mono dispersive)
pH range 0...14
Standard flow 1.0 mL/min
Temperatur range 10...70 °C