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Hamilton PRP-X300 - 250/4.0

6.1005.030 Hamilton PRP-X300 - 250/4.0
Exclusion column on polystyrene/divinylbenzene copolymer basis for determining organic acids.
 
6.1005.030 Hamilton PRP-X300 - 250/4.0
The Hamilton PRP-X300 ion-exclusion column is a cation-exchange column with a relatively low capacity. The combination of a styrene-divinylbenzene copolymer with sulfonic acid groups is ideal for the solution of simple separating problems. The possibility of determining the salts of organic acids and the extremely sensitive formate determination characterize this column.
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6.1005.030 Hamilton PRP-X300 - 250/4.0
Column dimensions (mm) 250 x 4.0
Max. pressure 34 MPa
Maximum flow 8.0 mL/min
Standard flow 1.0 mL/min
Temperature range (°C) 5...60
Housing material Stainless steel
Carrier material Polystyrene-divinylbenzene copolymer with sulfonic acid groups
Particle size (µm) 7
Column type 1
pH range 1...13
Storage For short times (days) in the eluent, for long times (weeks) in methanol/water (1:4)
Weight 0.11
Regeneration

Divalent cations remain on the column and form complexes with citrate that falsify the citrate peak. Injection of 100 µL 0.1 mol/L Na2H2EDTA.

Rinse the column with 0.01 mol/L H2SO4 with 20% methanol at a flow rate of 0.5 mL/min for 6 h.

Application range

Applications

  • glycolic acid, monochloroacetic acid
  • simple matrices
  • simple separating problems
  • formate determination
Capacity 19 (µmol K+ )
Organic modifier 0 - 100 %
Eluents

Perchloric acid eluent (standard eluent)

Perchloric acid: 1.5 mmol/L ; 30 mL/2 L (c = 0.1 mol/L)

Sulfuric acid eluent

Sulfuric acid: 0.5 mmol/L ; 10 mL/2 L (c = 0.1 mol/L)

6.1005.030 Hamilton PRP-X300 - 250/4.0
Options
6.1011.030 Metrosep RP 2 Guard/3.5
6.1011.130 Spare filter for RP 2 Guard/3.5, 10 pieces
6.1005.030 Hamilton PRP-X300 - 250/4.0
AN-O-002 Glycolic acid and monochloroacetic acid in cocoamidopropyl betaine
AN-O-003 Citrate and acetate in isotonic solutions
AN-O-020 Citric acid and lactic acid in an electroplating bath
AN-O-033 Glycolic and lactic acid in the presence of N-methyl pyrrolidone
AN-O-038

Separation of various sulfur/nitrogen compounds using ion-pair chromatography

AN-U-003 Glycolic and lactic acid in the presence of N-methyl pyrrolidone
6.1005.030 Hamilton PRP-X300 - 250/4.0
8.110.0002 Leaflet for 6.1005.030 IC Exclusion column PRP-X300, german/french/english